Published March 5, 1997
by Humana Press .
Written in English
|The Physical Object|
|Number of Pages||468|
Recombinant Protein Production in Yeast: Methods and Protocols examines the process of preparation of expression vectors, transformation to generate high-yielding clones, optimization of experimental conditions to maximize yields, scale-up to bioreactor formats and disruption of yeast cells to enable the isolation of the recombinant protein Author: Roslyn M. Bill. Authoritative and convenient, Recombinant Protein Expression in Mammalian Cells: Methods and Protocols aims to aid researchers in building on our knowledge of protein structure and function and to speed the discovery of new therapeutic proteins. Leading researchers and experts present wide-ranging methods for detecting and isolating expressed gene products-recombinant proteins. These state-of-the-art techniques describe a large number of molecular tags and labels, including enzymes, ligand-binding moieties, and immunodetectable molecules. Get this from a library! Recombinant protein protocols: detection and isolation. [Rocky S Tuan;] -- Leading researchers and experts present wide-ranging methods for detecting and isolating expressed gene products-recombinant proteins. These state-of-the-art techniques describe a large number of.
The first recombinant protein used in treatment was recombinant human insulin in The recombinant protein industry has rapidly grown. To date, more than recombinant proteins are approved by the US FDA for clinical use. However, more than recombinant proteins are . State-of-the-art and forward looking, Recombinant Gene Expression: Reviews and Protocols, Second Edition offers investigators seeking an overview of this critically important field not only the understanding, but also the tools needed to begin producing nonprotein products and altering the central metabolic pathways of cells to enhance. Recombinant Protein Expression in Mammalian Cells: Methods and Protocols David L. Hacker This detailed volume explores advances in vector design, DNA delivery, cell cultivation, host cell engineering, and bioprocess optimization within the study of recombinant protein expression in mammalian cells. Several practical protocols have been implemented with these high-cell-density expression methods to ensure a very high yield of recombinant protein production. With our methods and protocols, we routinely obtain 14–25 mg of NMR triple-labeled proteins and 17–34 mg of unlabeled proteins from a mL cell culture for all seven proteins we Cited by:
recombinant protein of interest such that the tag is used to capture or detect the protein. Here we provide guidelines on how to determine the best protein purification strategy and include protocols based on common affinity tags. We also describe popular tools and techniques for proteomics research. ng the Right Protein Purification. Pris: kr. Inbunden, Skickas inom vardagar. Köp Recombinant Protein Protocols av Rocky S Tuan på COVID Resources. Reliable information about the coronavirus (COVID) is available from the World Health Organization (current situation, international travel).Numerous and frequently-updated resource results are available from this ’s WebJunction has pulled together information and resources to assist library staff as they consider how to handle coronavirus. This ultimate guide book highlights the important tools and methods in experiments of cell culture and differentiation, proliferation, tissue regeneration, bioprocessing, assay development and other cytokine & protein research experiments. Download and expedite your research.